Two-dimensional crystallization of reaction centers from Chloroflexus aurantiacus |
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| Authors: | A N Barnakov V V Demin A P Kuzin A A Zargarov A S Zolotarev N G Abdulaev |
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| Affiliation: | Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow. |
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| Abstract: | Two-dimensional crystals of photosynthetic reaction centers from Chloroflexus aurantiacus were obtained from protein-lipid-detergent micelles by detergent dialysis. The size of crystals was up to 2 microns. Some of them were multilayered crystals. However, other crystal forms were also observed. Preliminary image processing analysis showed that crystals of one crystal form referred to two-sided plane group p2 and had the following unit cell parameters: a = 17.6 nm, b = 18.0 nm, gamma = 84 degrees. The contour map of the crystal stain-excluding region was calculated by the Fourier-filtering procedure at about 2 nm resolution. |
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