Synthesis of l-dopa by escherichia intermedia cells immobilized in a polyacrylamide gel |
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Authors: | G. Para J. Baratti |
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Affiliation: | (1) CNRS, Laboratoire de Chimie Bactérienne, Université de Provence, BP 71, F-13277 Marseille Cédex 9, France |
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Abstract: | Summary Escherichia intermedia cells were immobilized by entrapment in a polyacrylamide gel and used for l-dopa synthesis from pyrocatechol, pyruvate and ammonia. An immobilized cell preparation containing 75 mg cells/g gel retained 45%–50% of the activity of free cells. The effect of temperature, pH and substrate concentration of the initial rate of l-dopa synthesis was very similar for free and immobilized cells. Substrate inhibition was observed for pyrocatechol, pyruvate and ammonia. In a batch reactor, 5.4 g·l-1l-dopa was obtained, with 100% conversion yield of pyrocatechol and l-dopa productivity of 0.18 g·l-1·h-1. The use of a pyrocatechol-borate complex decreased by-product formation and catalyst inactivation. |
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