MicroRNA-214 Suppresses Oncogenesis and Exerts Impact on Prognosis by Targeting PDRG1 in Bladder Cancer |
| |
| Authors: | Jinfeng Wang Xin Zhang Lili Wang Yongmei Yang Zhaogang Dong Haiyan Wang Lutao Du Chuanxin Wang |
| |
| Affiliation: | 1Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China;2Department of Clinical Laboratory, Linyi People’s Hospital, Linyi, Shandong Province, China;Michigan State University, UNITED STATES |
| |
| Abstract: | MicroRNA-214 (miR-214) has been reported to be dysregulated in human bladder cancer tissues. We aimed to investigate the clinical correlation, biological significance and molecular network of miR-214 in bladder cancer. Our results showed miR-214 was down-regulated in bladder cancer tissues and significantly associated with tumor stage, lymph node status, grade, multifocality, history of non-muscle-invasive bladder cancer (NMIBC). Moreover, miR-214 could serve as an independent factor of recurrence-free survival (RFS) and overall survival (OS) for patients with muscle-invasive bladder cancer (MIBC). Restoration of miR-214 expression in bladder cancer cell lines inhibited cell proliferation, migration, invasion and markedly promoted apoptosis. Dual-luciferase reporter assay recognized PDRG1 as direct downstream target gene of miR-214. PDRG1 was significantly increased in tumors low of miR-214 and knockdown of PDRG1 mimicked the effects of miR-214 overexpression. Our findings manifest that miR-214 could exert tumor-suppressive effects in bladder cancer by directly down-regulating oncogene PDRG1 and suggest an appealing novel indicator for prognostic and therapeutic intervention of bladder cancer. |
| |
| Keywords: | |
|
|
