| Abstract: | The minor component of the human adult hemoglobin Hb A2 (alpha2/delta2) was isolated. The peptide chains were separated by counter current distribution, the delta-chains digested with trypsin and all hydrolysis splitting products separated and quantitatively characterized. With the film technique the different peptides were sequenced with hydrophilic phenylisothiocyanates I and IV or by the propyne programme. The complete sequence of the delta-chains is given and compared with the sequence reported in former investigations, in which the delta-chain sequence was only partly elucidated. Having completely sequenced the delta-chains of the human hemoglobin, we found the 10 previously reported exchanges and no additional exchanges with respect to the beta-chains. |
