Sensitized guinea-pig lung: altered adenylate cyclase stimulation by epinephrine |
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Authors: | A A Mathé S Puri L Volicer |
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Institution: | Laboratory of Biogenic Amines and Allergy, Departments of Psychiatry and Pharmacology, Boston University School of Medicine, Boston, Massachusetts 02118, USA |
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Abstract: | Adenylate cyclase (AC) was measured in healthy and sensitized quinea-pig lungs. Basal activities were 24.49 ± 2.50 and 26.73 ± 3.03 pmols cyclic AMP mg protein/minute, respectively. NaF produced about threefold activity increase in both groups. Low concentrations of epinephrine (EPI) 10?9 ? 10?6M, maximally stimulated the enzyme in sensitized lungs. In contrast, these concentrations had no effect in healthy lungs. Higher EPI concentrations, 10?5 ? 10?2M, while progressively stimulating less the AC in sensitized lungs, increased the response in the healthy lungs. The maximal increase in AC activity, about 200%, was achieved with 10?6 and 10?3M EPI in sensitized and healthy lungs, respectively. Propranolol blocked the effect of EPI in both groups. The results indicate that sensitization altered the AC system in guinea-pig lungs. |
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