Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display |
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Authors: | Yamazaki Takayuki Aoki Satoko Ohta Keisuke Hyuma Shinji Sakaguchi Kengo Sugawara Fumio |
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Institution: | Genome and Drug Research Center, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan. |
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Abstract: | SQAG9, a new class of immunosuppressive sulfoquinovosylacylglycerol, and its biotinylated derivatives have been synthesized. A T7 Phage library, composed of random cDNA fragments from Drosophila melanogaster, displayed a possible binding peptide of 14 amino acids. The immobilized synthetic peptide on a sensor chip showed a dissociation constant of K(D)=1.5 x 10(-6) against SQAG9 in a surface plasmon resonance experiment. |
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