Immunological assays for chemokine detection in in-vitro culture of CNS cells |
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| Authors: | Supriya?D?Mahajan Stanley?A?Schwartz Email author" target="_blank">Madhavan?P?N?NairEmail author |
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| Institution: | (1) Department of Medicine, Division of Allergy, Immunology, and Rheumatology and State University of New York and Buffalo General Hospital, Kaleida Health 100 High Street, 14203 Buffalo, NY;(2) Department of Medicine, Division of Allergy, Immunology and Rheumatology, 301 Multi Research Bldg., Buffalo General Hospital, 100 High Street, 14203 Buffalo, NY |
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| Abstract: | Herein we review the various methods currently in use for determining the expression of chemokines by CNS cells in vitro.
Chemokine detection assays are used in conjuction with one another to provide a comprehensive, biologically relevant assessment
of the chemokines which is necessary for correct data interpretation of a specific observed biological effect. The methods
described include bioassays for soluble chemokine receptors, RNA extraction, RT-PCR, Real — time quantitative PCR, gene array
analysis, northern blot analysis, Ribonuclease Protection assay, Flow cytometry, ELISPOT, western blot analysis, and ELISA.
No single method of analysis meets the criteria for a comprehensive, biologically relevant assessment of the chemokines, therefore
more than one assay might be necessary for correct data interpretation, a choice that is based on development of a scientific
rationale for the method with emphasis on the reliability and relevance of the method.
Published: April 7, 2003 |
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