不结球白菜抗根肿病渐渗系的分子辅助选育

该研究基于与大白菜抗根肿病连锁的分子标记,设计特异引物,获得简便实用的SCAR标记,并用于分子标记辅助选择,创制不结球白菜抗根肿病新材料。结果发现,在设计的8对特异引物中,有1对特异引物在抗、感亲本间表现出多态性。F2群体验证发现,该标记与已有SSR标记及根肿病抗性共分离,能够用于抗根肿病鉴定,定名为CRb-R-25。通过亚种间杂交并回交,利用标记CRb-R-25辅助选择将大白菜根肿病抗性转入不结球白菜中,获得抗根肿病不结球白菜渐渗系材料TQ14-1-15。

Marker-assisted Breeding of Clubroot Resistant Introgression Line in Non-heading Chinese Cabbage

We designed SCAR markers according to the markers linked to clubroot resistant gene in Chinese cabbage,in order to develop the new clubroot resistant materials of non-heading Chinese cabbage through marker-assisted selection (MAS).The results indicated that one pair primer among 8 showed polymorphism between parents of resistant and susceptible,and co-segregation with reported SSR marker and resistant characterization of F₂,suggested that it could be used to identify the clubroot resistance,named CRb-R-25.Based on the inter-subspecies hybrid,backcross and selection of CRb-R-25,TQ14-1-15 with clubroot resistance of non-heading Chinese cabbage was bred by introgression of resistance gene in Chinese cabbage.