| DNA稳定的银纳米簇诱导的双酶催化转化检测研究 |
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| 引用本文： | 邓君,邱波.DNA稳定的银纳米簇诱导的双酶催化转化检测研究[J].氨基酸和生物资源,2015(2):68-74. |
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| 作者姓名： | 邓君 邱波 |
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| 作者单位： | 武汉大学人民医院，骨科，湖北 武汉 430060 |
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| 摘 要： | 为了实现对碱性磷酸酶和邻-磷酸-L-酪氨酸的同时检测，本研究利用 DNA 稳定的银纳米簇（AgNCs／DNA）诱导的生物催化转化，基于醌类物质对 DNA 稳定的银纳米簇的荧光的猝灭效应，通过酪氨酸酶能与酪胺、多巴胺以及酪氨酸反应生成醌类物质，间接地达到了对酪胺、多巴胺以及酪氨酸的定量检测。AgNCs／DNA 还被进一步用于双酶催化级联反应，通过碱性磷酸氧化酶（ALP）和酪氨酸酶（TYR）组成的双酶系统，实现了对碱性磷酸酶和邻-磷酸-L-酪氨酸的高灵敏度检测，检测限分别达到2．5×10－5μmol·L－1和0．01μmol·L－1。
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| 关 键 词： | 银纳米簇 荧光 酪氨酸酶 碱性磷酸酶 邻-磷酸-犔-酪氨酸 |
Detection of Double Enzyme Catalyzed Conversion with Luminescent DNA/Silver Nanoclusters |
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| DENG Jun,QIU Bo.Detection of Double Enzyme Catalyzed Conversion with Luminescent DNA/Silver Nanoclusters[J].Amino Acids & Biotic Resources,2015(2):68-74. |
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| Authors: | DENG Jun QIU Bo |
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| Abstract: | In order to realize the detection of alkaline phosphatase and o-phospho-L-tyrosin,in this study,using DNA stability of silver nanoclusters (AgNCs/DNA)induced biological catalytic conversion,based on quinone material for DNA stable nanometer silver clusters of fluorescence quenching effect,the quantitative detection of tyramine,do-pamine and tyrosine was perfermed.AgNCs/DNA was further used to the double enzyme catalytic cascades.Alkaline phosphate oxidase (ALP)and tyrosinase (TYR)were used as double enzyme system,and high sensitivity detection of alkaline phosphatase and o-phospho-L-tyrosin was achived.The detection limit reached to 2.5 × 10-5μmol·L-1 and 0.01 μmol·L-1 respectively. |
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| Keywords: | silver nanoclusters fluorescence tyrosinase alkaline phosphatase o-phospho-L-tyrosin |
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