Synthesis of 25-hydroxy[26,27-3h]vitamin D3 with high specific activity. |
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| Authors: | J L Napoli M A Fivizzani A J Hamstra H K Schnoes H F DeLuca |
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| Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, 420 Henry Mall, Madison, Wisconsin 53706 USA |
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| Abstract: | A synthesis of radiochemically pure 25-hydroxy26,27-3H]vitamin D3 with a specific activity of 160 Ci/mmol is reported. The structure and biological activity of the radiolabeled compound was verified by comigration on high-pressure liquid chromatography with synthetic 25-hydroxyvitamin D3 to constant specific activity, and by conversion in vitro to 1α,25-dihydroxy26,27-3H]vitamin D3 with the chick kidney 1α-hydroxylase. |
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