Cyclodextrins for growth ofHelicobacter pylori and production of vacuolating cytotoxin |
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| Authors: | Antonio Marchini Maria d’Apolito Paola Massari Marco Atzeni Michael Copass Roberto Olivieri |
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| Affiliation: | (1) The Biocine Immunobiological Research Institute Siena, IRIS, Via Fiorentina 1, 1-53100 Siena, Italy;(2) The Institute of Biochemistry and Enzymology, University of Siena, Via dei Mantellini 44, I-53100 Siena, Italy |
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| Abstract: | Growth ofHelicobacter pylori in liquid culture requires the addition of media supplements that often interfere with subsequent purification of bacterial antigens. In order to determine whether cyclodextrins can substitute for conventionalH. pylori growth supplements, we culturedH. pylori in the presence of five commercially available cyclodextrins. The effect of these compounds on the production of the vacuolating cytotoxin antigen was evaluated. Several cyclodextrins supported flourishing growth and permitted the consistent production of vacuolating cytotoxin. These data suggest that Brucella broth supplemented with cyclodextrins is an improved medium for bacterial culture and industrial production ofH. pylori antigens. |
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| Keywords: | Helicobacter pylori growth medium Vacuolating cytotoxin Cyclodextrins |
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