Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825 and activating defense-related genes in Arabidopsis |
| |
Authors: | Dongdong Niu Jing Xia Chunhao Jiang Beibei Qi Xiaoyu Ling Siyuan Lin Weixiong Zhang Jianhua Guo Hailing Jin Hongwei Zhao |
| |
Affiliation: | 1.Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, China;2.Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, California, CA-92521, USA;3.Department of Computer Science and Engineering, Washington University in St Louis, St Louis, MO 63130, USA;4.The Institute for Systems Biology, Jianghan University, Wuhan 430056, China |
| |
Abstract: | Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance(ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here,by comparing small RNA profiles of Pseudomonas syringae pv. tomato(Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis micro RNAs(mi RNAs) that are differentially regulated by AR156 pretreatment. mi R825 and mi R825 are two mi RNA generated from a single mi RNA gene.Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. mi R825 targets two ubiquitin-protein ligases,while mi R825 targets toll-interleukin-like receptor(TIR)-nucleotide binding site(NBS) and leucine-rich repeat(LRR)type resistance(R) genes. The expression of these target genes negatively correlated with the expression of mi R825 and mi R825. Moreover, transgenic plants showing reduced expression of mi R825 and mi R825 displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing mi R825 and mi R825 were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing mi R825 and mi R825 and activating the defense related genes they targeted. |
| |
Keywords: | Induced systemic resistance ISR microRNA plant innate immunity small RNA |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《植物学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《植物学报(英文版)》下载全文 |
|