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Immunohistochemical study of creatine kinase isozymes in the human upper limb bud
Authors:H Shinohara  M Oguni  O Tanaka  K Kato
Institution:Department of Anatomy, Shimane Medical University, Izumo, Japan.
Abstract:Creatine kinase (CK) is involved in the production of ATP and is composed of two immunologically distinct subunits, B (CK-B) and M (CK-M). In the differentiation of myoblasts, the isozyme of CK changes from CK-B to CK-M. In the present study, the expression of CK subunits was studied immunohistochemically in the upper limb bud of human embryos (Carnegie stages 13-21). It was found that CK-B and CK-M immunoreactive cells appeared at stage 15 and at stage 18, respectively.
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