Germination and Dormancy of Abscisic Acid- and Gibberellin-Deficient Mutant Tomato (Lycopersicon esculentum) Seeds (Sensitivity of Germination to Abscisic Acid,Gibberellin, and Water Potential)

Germination responses of wild-type (MM), abscisic acid (ABA)-deficient (sitw), and gibberellin (GA)-deficient (gib-1) mutant tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds to ABA, GA4+7, reduced water potential (psi]), and their combinations were analyzed using a population-based threshold model (B.R. Ni and K.J. Bradford 1992] Plant Physiol 98: 1057-1068). Among the three genotypes, sitw seeds germinated rapidly and completely in water, MM seeds germinated more slowly and were partially dormant, and gib-1 seeds did not germinate without exogenous GA4+7. Times to germination were inversely proportional to the differences between the external osmoticum, ABA, or GA4+7 concentrations and the corresponding threshold levels that would either prevent (psi]b, logABAb]) or promote (logGAb]) germination. The sensitivity of germination to ABA, GA4+7, and psi] varied widely among individual seeds in the population, resulting in a distribution of germination times. The rapid germination rate of sitw seeds was attributable to their low mean psi]b (-1.17 MPa). Postharvest dormancy in MM seeds was due to a high mean psi]b (-0.35 MPa) and a distribution of psi]b among seeds such that some seeds were unable to germinate even on water. GA4+7 (100 mu]M) stimulated germination of MM and gib-1 seeds by lowering the mean psi]b to -0.75 MPa, whereas ABA inhibited germination of MM and sitw seeds by increasing the mean psi]b. The changes in psi]b were not due to changes in embryo osmotic potential. Rather, hormonal effects on endosperm weakening opposite the radicle tip apparently determine the threshold psi] for germination. The analysis demonstrates that ABA- and GA-dependent changes in seed dormancy and germination rates, whether due to endogenous or exogenous growth regulators, are based primarily upon corresponding shifts in the psi] thresholds for radicle emergence. The psi] thresholds, in turn, determine both the rate and final extent of germination within the seed population.