薄荷精油抗菌活性研究

采用气相色谱—质谱联用仪分析了薄荷精油的成分，在鉴定出的21种成分中，异佛尔酮(41.22%)、β-石竹烯(10.01%)、斯巴醇(2.89%)、β-蒎烯(1.45%)和桉树脑(1.13%)为主要成分。采用琼脂扩散法和微量肉汤稀释法对薄荷精油的抗菌活性进行了研究。结果表明，枯草芽孢杆菌及变形杆菌出现最大的抑菌环。MIC实验中，薄荷精油的浓度范围为5.00%~0.039%。在所选的8种菌种中，变形杆菌的MIC及MBC值最低，分别为0.625%及1.25%。结果充分证明，薄荷精油对于所选的8种病原菌都有很好的抗菌活性，在食品及制药工业中有广阔的应用前景。

Antimicrobial Activities of Mentha haplocalyx Briq.Essential Oil

The sample of the Mentha haplocalyx Briq. essential oil was analyzed by GC-MS. From 21 compounds representing the oils, Isophorone(41.22%), β-Caryophyllene(10.01%), Spathulenol(2.89%), β-Pinene(1.45%) and Eucalyptole(1.13%) appeared as the main components. The screening of antimicrobial activity of the essential oil was evaluated using agar diffusion and broth microdilution methods. Tests showed that the oil presented the biggest inhibition zones in the two strains of Bacillus subtilis and Proteus vulgaris. The MICs of the oil against the tested microorganisms ranged from 5.00% to 0.039%. Of the 8 microorganisms, Proteus vulgaris presented the lowest MIC(0.625%) and MBC(1.25%) values. Results presented here may suggest that the essential oil of Mentha haplocalyx Briq. possesses antimicrobial properties, and therefore is a potential source of antimicrobial ingredients for food and pharmaceutical industry.