A dihydrofolate reductase gene from Candida albicans: molecular cloning |
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| Authors: | M. Franceschi M. Denaro T. Irdani R. Lorenzetti G. Mastromei E. Skarmoutsou M. Polsinelli |
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| Affiliation: | Department of Animal Biology and Genetics, University of Firenze, Italy. |
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| Abstract: | The dihydrofolate reductase gene from Candida albicans has been cloned and partially characterized. A genomic bank from C. albicans strain 10127/5 was constructed in Escherichia coli and screened for trimethoprim resistance. A plasmid pMF1, carrying the resistance marker was isolated and characterized by restriction mapping and Southern blotting. Cells harbouring pMF1 were as sensitive as the parental cells to a wide spectrum of antibacterial agents, except for trimethoprim; the dihydrofolate reductase activity from these cells was trimethoprim resistant. |
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| Keywords: | DHFR Candida albicans Gene cloning Bacterial expression |
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