The intracellular distribution of tocopherols in Calendula officinalis leaves

From Calendula officinalis leaves, five cellular subtractions (chloroplasts, mitochondria, Golgi membranes, microsomes and cytosol) were obtained and their purity was checked. The contents of α-,γ- and δ-tocopherols were determined in these fractions. There were no tocopherols in Golgi membranes and cytosol. γ-Tocopherol and δ-tocopherol were found in the chloroplasts, mitochondria and microsomes, whereas α-tocopherol was present only in the chloroplasts.