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Physical mapping and molecular-cytogenetic analysis of substitutions and translocations involving chromosome 1D in synthetic hexaploid triticale
Authors:U Hohmann  J Zoller  R G Herrmann  M E Kazman
Institution:Botanisches Institut der Ludwig-Maximilians-Universit?t München, Menzinger Str. 67, D-80638 München, Germany Fax: +49-89-178 22 74 E-mail: uhemail@botanik.biologie.uni-muenchen.de, DE
Institut für Pflanzenbau und Pflanzenzüchtung, Georg-August Universit?t G?ttingen, Von-Siebold-Str. 8, D-37075 G?ttingen, Germany, DE
Abstract: Chromosome 1D, which carries the advantageous alleles of glutenin and gliadin, attracts major interest with respect to improving the bread-making quality of triticale. Eighty-one BC1F4 lines from different primary and secondary hexaploid triticale crosses were selected for 1D chromatin analysis using SDS-PAGE and C-banding. In situ hybridization and RFLP-based comparative physical mapping of group 1 chromosomes revealed 20 lines with complete 1D (1A) substitutions. Nine 1D (1B) substitutions, six 1D (1R) substitutions and one 1D addition line were also selected. Three lines were pure AABBRR hexaploids without any D-genome chromosomes. For the remaining 42 lines (51.8%), a wide spectrum of 20 different recombinations between chromosomes 1A and 1D was uncovered. Altogether, they were generated without any earlier irradiation, tissue culture or genetic induction of chromosome pairing. In addition, 14 translocations between 1B/1D, 1A/1R, 1B/1R, 1D/1R and 1A/1B were detected. Considerable variability for sedimentation values was found, with the highest sedimentation values among lines with complete 1D chromosomes. The implications of using triticale as a model for generating compensating chromosome rearrangements in defined homoeologous groups and the breeding potential of D-genome chromatin introgressed into triticale with improved sedimentation values are discussed. Received: 27 July 1998 / Accepted: 5 August 1998
Keywords:  Physical map  Synteny  FISH  C-banding  Homoeologous recombination
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