Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat |
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Authors: | Yoshioka Hiroyuki Ohmoto Tsuyoshi Urisu Atsuo Mine Yoshinori Adachi Taiji |
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Institution: | Graduate School of Agricultural and Biological Sciences, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka, Japan. |
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Abstract: | Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive substances but also allergenic proteins that cause hypersensitive reactions. Thus, the development of hypoallergenic buckwheat would make this important pseudo-cereal available to allergic people. A major allergenic protein of buckwheat is Fag e 1. We isolated the respective cDNA, coding for a 22 kDa protein, from a recently developed autogamous strain of common buckwheat and confirmed its immunoglobulin E (IgE)-binding activity using recombinant Fag e 1 and sera of allergic patients. The derived amino acid sequence from Fag e 1 cDNA was used to synthesize an overlapping peptide library on nitrocellulose membranes for the determination of the Fag e 1 epitopes. We identified eight epitopes and the critical amino acids for IgE-binding within the epitopes. This epitope analysis of a major allergenic protein of buckwheat should help therapeutic efforts and aid in the development of hypoallergenic buckwheat. |
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