肺腺癌预后相关miRNA生物信息学筛选及其临床意义

目的：探讨肺腺癌预后相关miRNA组学特征及其临床意义。方法：应用癌症基因组图谱（TCGA）数据库，检索人肺腺癌miRNA表达谱数据，进行差异分析，再利用Cox风险回归模型筛选预后相关miRNA；利用mirwalk分析平台，对筛选出的miRNA进行靶向调控基因预测、KEGG功能富集分析，最后，预测出预后相关miRNA的功能。结果：共筛选肺腺癌差异miRNA46个，其中，上调19个、下调27个；通过Cox生存分析筛选到预后相关miRNA有6个，即hsa-mir-21、hsa-mir-142、hsa-mir-200a高表达，hsa-mir-101、hsa-let-7c、hsa-mir-378e低表达，其中hsa-mir-21、hsa-mir-378e与肺腺癌患者不良预后有关，生存期显著缩短（P<0.05，AUC=0.618）。KEGG分析上述预后相关miRNA靶向调控基因与免疫反应通路、miRNA与癌症通路、代谢通路等有关。结论：hsa-mir-21、hsa-mir-378e与肺腺癌预后不良有关，未来经进一步临床验证有可能作为肺腺癌预后相关的分子标记物。

Prognosis-related miRNA bioinformatics screening of lung adenocarcinoma and its clinical significance

Objective: To investigate the prognosis-related miRNA histological features and clinical significance of lung adenocarcinoma. Methods: Using The Cancer Genome Atlas (TCGA) data, the miRNA expression profile data of human lung adenocarcinoma were searched for differential analysis, and the prognosis-related miRNAs were screened by Cox risk regression model. The targeted miRNAs were predicted by mirwalk analysis platform, KEGG functional enrichment analysis, and finally, predict the function of prognosis-related miRNAs. Results: A total of 46 differential miRNAs in lung adenocarcinoma were screened, including 19 up-regulated and 27 down-regulated. Six prognostic-related miRNAs were screened by Cox survival analysis, namely hsa-mir-21, hsa-mir-142, hsa-mir-200a high expression, hsa-mir-101, hsa-let-7c, hsa-mir-378e low expression, hsa-mir-21 and hsa-mir-378e were associated with poor prognosis in patients with lung adenocarcinoma, and the survival time was shortened significantly (P<0.05, AUC=0.618). KEGG analysis showed that the above prognosis-related miRNA targeting regulatory genes were related with immune response pathways, miRNA and cancer pathways, metabolic pathways and so on. Conclusion: Hsa-mir-21 and hsa-mir-378e are associated with poor prognosis of lung adenocarcinoma, and may be used as a molecular marker for prognosis of lung adenocarcinoma after further clinical verification.