Quantitative DNA hybridization in solution using magnetic/luminescent core-shell nanoparticles |
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Authors: | Son Ahjeong Dosev Dosi Nichkova Mikaela Ma Zhiya Kennedy Ian M Scow Kate M Hristova Krassimira R |
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Affiliation: | Department of Land, Air, and Water Resources, University of California Davis, One Shields Avenue, Davis, CA 95616, USA. |
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Abstract: | Nanoscale magnetic/luminescent core-shell particles were used for DNA quantification in a hybridization-in-solution approach. We demonstrated a rapid, simple, and non-polymerase chain reaction-based DNA hybridization-in-solution assay for quantifying bacteria capable of biodegrading methyl tertiary-butyl ether. Fe3O4/Eu:Gd2O3 core-shell nanoparticles synthesized by spray pyrolysis were biofunctionalized with NeutrAvidin. Following immobilization of a biotinylated probe DNA on the particles' surfaces via passive adsorption, target DNA labeled with fluorescein isothiocyanate was hybridized with probe DNA. The hybridized DNA complex was separated from solution with a magnet, while nonhybridized DNA remained in solution. The normalized fluorescence (fluorescein isothiocyanate/nanoparticles) measured with a spectrofluorometer indicated a linear quantification (R(2)=0.98) of the target bacterial 16 S rDNA. The rate of hybridization increased concurrently with the target DNA concentration. In addition, this approach differentiated between the signal outputs from perfectly complementary target and two-base mismatched target DNA in a range of concentrations, showing the specificity of the assay and the possibility for environmental applications. |
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Keywords: | DNA Hybridization-in-solution Nanoparticles Methyl tertiary-butyl ether Lanthanide oxide Magnetic particles |
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