Release and exchange of neurotransmitters in synaptosomes: Effects of the ionophore A23187 and of ouabain |
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Authors: | Giulio Levi Peter J. Roberts Maurizio Raiteri |
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Affiliation: | (1) Laboratorio di Biologia Cellulare, CNR, Rome;(2) Department of Physiology and Biochemistry, University of Southhampton, England;(3) Istituto di Farmacologia, Università Cattolia, Rome |
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Abstract: | The effects of the ionophore A23187 and of ouabain on the release of [3H]GABA and [3H]norepinephrine were studied in superfused rat brain synaptosomes. Each of the two drugs moderately stimulated the spontaneous release of [3H]GABA, but greatly potentiated the release of [3H]GABA induced by unlabeled GABA. In contrast, the ionophore and norepinephrine showed an additive, but not a supraadditive, releasing effect on synaptosomal [3H]norepinephrine. Ouabain modestly and transiently potentiated the norepinephrine-induced [3H]norepinephrine release, which, however, was inhibited by the drug after a few minutes. It is suggested that in the new intrasynaptosomal ionic conditions determined by the two drugs, the stoichiometry of the basal homoexchange of GABA is changed in a direction favoring net outward transport. |
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