Effect of sesamol on radiation-induced cytotoxicity in Swiss albino mice |
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Authors: | Vipan Kumar Parihar K.R. Prabhakar Veeresh P. Veerapur M. Sudheer Kumar Y. Rosi Reddy Ravi Joshi M.K. Unnikrishnan C. Mallikarjuna Rao |
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Affiliation: | aDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 576104, India;bDepartment of Radiation and Photochemistry, Bhabha Atomic Research Center, Trombay, Mumbai, India |
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Abstract: | The radio-protective ability of sesamol (SM) at various doses viz., 0, 10, 25, 40, 50, 70 and 100 mg/kg bw, administered intraperitoneally 30 min prior to 9.5 Gy whole-body γ-irradiation was studied in Swiss albino mice. Radiation toxicity and mortality were observed during a period of 30 days and the percentage mortality was calculated. SM pretreatment with 50 mg/kg bw was found to be the most effective dose in maintaining body weight and in reducing the percentage mortality, while 100 mg/kg bw was found to be more effective in maintaining the spleen index and in stimulation of endogenous spleen colony-forming units. Pretreatment with SM (50 mg/kg bw) in mice irradiated with 15 Gy significantly reduced dead, inflammatory, mitotic and goblet cells in irradiated jejunum. SM at 50 mg/kg bw also increased crypt cells, maintained villus height, and prevented mucosal erosion. Nuclear enlargement in epithelial cells was found less in SM-treated mice compared with the irradiated control. The radiation-induced decrease in endogenous antioxidant enzymes (GSH, GST, catalase) and the increase in lipid peroxidation were also reduced by pretreatment with SM [50 and 100 mg/kg bw] at all monitored post-irradiation intervals. There was no protection at a dose less than 25 mg/kg bw. |
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Keywords: | Sesamol Radioprotection Jejunum Spleen index Glutathione Catalase Total thiol GST Lipid peroxidation |
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