Identification of genetic markers for Korean native cattle (Hanwoo) by RAPD analysis |
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Authors: | Jung Sou Yeo Ji Sun Lee Chang Hee Lee Young Ja Jung Doo Hyun Nam |
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Institution: | (1) Department of Animal Sciences, College of Natural Resources, Yeungnam University, 712-749 Kyongsan, Korea;(2) Division of Pharmacy, College of Pharmacy, Yeungnam University, 712-749 Kyongsan, Korea;(3) Korea Food and Drug Administration, Eunpyung-ku, 122-704 Seoul, Korea |
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Abstract: | In order to develop the specific genetic marker for Korean native cattle (Hanwoo), randomly amplified polymorphic DNA (RAPD)
analysis of 6 different cattle breeds was attempted by using 38 decamer primers. In comparison of RAPD patterns, two distinctive
DNA bands specific for Hanwoo were detected. One was 296 bp of DNA fragment found to be specific only for female Hanwoo when
primer GTCCACACGG was employed. In individual analysis of this RAPD marker was observed only in female individuals with the
possibility of 85.3%. The other was 521 bp of RAPD marker amplified using TCGGCGATAG and AGCCAGCGAA primers, which showed
83.0% of genetic frequency in 85 male and 68 female individuals tested. Nucleotide sequencing of these genetic markers revealed
that 296 bp marker has a short microsatellite-like sequence, ACCACCACAC, and a tandem repeat sequence of microsatellite GAAAAATG
in the determined sequence. Two distinctive tandem repeats of microsatellite sequences, AAC and GAAGA, were also appeared
in 521 bp DNA marker. In BLAST search, any gene having high homology with these markers was not found |
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Keywords: | RAPD marker Korean native cattle Hanwoo DNA polymorphism genetic analysis |
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