cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas |
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Authors: | Itoh Naoki Takahashi Keisuke G |
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Institution: | Aquacultural Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiya-machi, Aoba-ku, Sendai, Miyagi 981-8555, Japan. |
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Abstract: | A novel lysozyme cDNA from the Pacific oyster, Crassostrea gigas, was identified. This second lysozyme from the Pacific oyster was designated as CGL-2. The complete CGL-2 cDNA sequence comprises of 536 bp, and 429 bp of the open reading frame encodes 147 bp of amino acid residues. Estimated CGL-2 molecular characteristics (isoelectric point and numbers of peptide recognition sites) resembled those of cv-lysozyme 2, a digestive lysozyme of the eastern oyster, Crassostrea virginica. Moreover, CGL-2 is phylogenetically homologous to the cv-lysozyme 2, indicating that CGL-2 and cv-lysozyme 2 evolved from the same ancestor protein for adaptation to the digestive environment. In situ hybridization revealed that the CGL-2 gene is expressed in digestive cells. It is noteworthy that the other Pacific oyster lysozyme, CGL-1, was also transcribed in the same cells. Presence and expression of multiple lysozymes in the digestive diverticula suggest that CGL-1 and CGL-2 might play complementary roles in digestive organs. |
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