The terminal respiratory chain of the methylotrophic bacterium Methylophilus methylotrophus |
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Authors: | M A Carver C W Jones |
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Affiliation: | Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England |
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Abstract: | Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypeptides; the complex exhibits a high ascorbate-TMPD oxidase activity which is inhibited non-competitively by cyanide (Ki approximately 2 microM). In contrast, the oxidation of methanol by whole cells is inhibited uncompetitively by cyanide (Ki approximately 4 microM), thus indicating the involvement in methanol oxidation of cytochrome oxidase aa3 rather than o. |
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Keywords: | Methanol Respiratory chain HQNO median inhibitory dose SDS sodium dodecylsulphate TMPD To whom correspondence should be addressed |
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