首页 | 本学科首页   官方微博 | 高级检索  
     


The terminal respiratory chain of the methylotrophic bacterium Methylophilus methylotrophus
Authors:M A Carver  C W Jones
Affiliation:Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England
Abstract:Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypeptides; the complex exhibits a high ascorbate-TMPD oxidase activity which is inhibited non-competitively by cyanide (Ki approximately 2 microM). In contrast, the oxidation of methanol by whole cells is inhibited uncompetitively by cyanide (Ki approximately 4 microM), thus indicating the involvement in methanol oxidation of cytochrome oxidase aa3 rather than o.
Keywords:Methanol  Respiratory chain  HQNO  median inhibitory dose  SDS  sodium dodecylsulphate  TMPD  To whom correspondence should be addressed
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号