A novel method of quantifying root exudation in the presence of soil microflora |
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Authors: | A A Meharg K Killham |
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Institution: | (1) Department of Plant and Soil Science, University of Aberdeen, AB9 2UE Aberdeen, Scotland;(2) Department of Biological Sciences, Hatherly Laboratories, Prince of Wales Road, EX4 4PS Exeter, UK |
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Abstract: | A microcosm is described in which root exudation may be estimated in the presence of microorganisms. Ryegrass seedlings are grown in microcosms in which roots were spatially separated from a microbial inoculant by a Millipore membrane. Seedlings grown in the microcosms were labelled with 14C]-CO2, and the fate of the label within the plant and rhizosphere was determined. Inoculation of the microcosms with Cladosporium resinae increased net fixation of the 14C] label compared to plants grown under sterile conditions. Inoculation also increased root exudation. The use of the microcosm was illustrated and its applications discussed. |
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Keywords: | Lolium perenne rhizosphere root exudation |
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