| 云南松花粉中钙调素结合蛋白的分离和鉴定 |
| |
| 引用本文： | 龚明,杨中汉,曹宗巽. 云南松花粉中钙调素结合蛋白的分离和鉴定[J]. 植物生理与分子生物学学报, 1994, 0(4) |
| |
| 作者姓名： | 龚明 杨中汉 曹宗巽 |
| |
| 作者单位： | 北京大学生物系，云南师范大学生物系 |
| |
| 摘 要： | 通过ＣａＭ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析方法从云南松花粉中提取出１０种ＣａＭ结合蛋白。它们均能抑制ＣａＭ对ＰＤＥ的激活，但这种抑制可被随后加入的过量的ＣａＭ所消除。酶活测定表明ＣａＭ结合蛋白中有Ｃａ２＋－依赖的ＡＴＰａｓｅ活力，但无植酸酶、过氧化物酶、酸性磷酸酶和磷脂酶Ｄ活性。
|
| 关 键 词： | 钙调素结合蛋白，ATP酶，花粉，云南松 |
Isolation and Identification of Calmodulin-Binding Proteins from Pinus yunanensis Pollen |
| |
| GONG Ming,YANG Zhong-Han and CAO Zong-Xun. Isolation and Identification of Calmodulin-Binding Proteins from Pinus yunanensis Pollen[J]. Journal Of Plant Physiology and Molecular Biology, 1994, 0(4) |
| |
| Authors: | GONG Ming YANG Zhong-Han CAO Zong-Xun |
| |
| Abstract: | Ten CaM-binding proteins were isolsted from Pinus yunnanensis pollen by CaM-Sepharose 4B affinity chromatography. These proteins could inhibit.CaM-activated PDE activity, which could be eliminated by adding an excess of exogenous CaM.The assay of enzyme activity indicated that there were Ca2 - and CaM-dependent ATPase activities,but no peroxidese, phytase,acid phosphatase and phospholipaSe D activities in CaM-binding proteins of pine pollen. |
| |
| Keywords: | CaM-binding proteins ATPase Pollen Pinus yunnanensis |
| 本文献已被 CNKI 等数据库收录！ |
