Impala,a transposon from Fusarium oxysporum,is active in the genome of Penicillium griseoroseum |
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Authors: | de Queiroz Marisa Vieira Daboussi Marie Josée |
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Institution: | 1. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China;2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China;3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China |
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Abstract: | An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production. |
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Keywords: | Transposon impala Heterologous transposition Filamentous fungus Penicillium griseoroseum |
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