Characterization of a glucuronyltransferase: neolactotetraosylceramide glucuronyltransferase from rat brain |
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Authors: | Chika Kawashima Koji Terayama Masayuki II Shogo Oka Toshisuke Kawasaki |
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Affiliation: | (1) Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, 606-01 Kyoto, Japan |
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Abstract: | The properties of a rat brain glucuronyltransferase, which is presumed to be associated with the biosynthesis of the HNK-1 epitope on sulfoglucuronyl glycolipids, are described. The enzyme required divalent cations for reaction, with maximal activity at 10mm Mn2+, and exhibited a dual optimum at pH 4–5 and pH 6 depending upon the buffer used, with the highest activity at pH 4.5 in MES buffer. This enzyme strictly recognized the Gal1-4GlcNAc terminal structure, and was highly specific for neolacto (type 2) glycolipids as acceptor. The enzyme was localized specifically in the brain, and was barely detected in other issues, including the thymus, spleen, liver, kidney, lung, and sciatic nerve fibres. Phosphatidylinositol and phosphatidylserine increased the enzymatic reaction 4.4- and 2.3-fold, respectively, whereas phosphatidylcholine slightly decreased the rate.Abbreviations GlcA glucuronic acid - Lc-PA14 lactotetraose-phenyl-C14H29 - nLc-PA14 neolactotetraose-phenyl-C14H29 - nLcOse4-Cer neolactotetraosylceramide - NP-40 Nonidet P-40 - PC phosphatidylcholine - PE phosphatidylethanolamine - PI phosphatidylinositol - PS phosphatidylserine - SGGL sulfoglucuronyl glycolipid |
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Keywords: | glucuronyltransferase HNK-1 antigen rat brain |
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