Radioimmunoassays for androsterone, 5alpha-androstane-3alpha, 17beta-diol and 5alpha-androstane-3beta, 17beta-diol |
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| Authors: | J Zamecnik G Barbe W H Moger D T Armstrong |
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| Institution: | College of Pharmacy and Upjohn Center for Clinical Pharmacology The university of Michigan Ann Arbor, Michigan 48109 USA |
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| Abstract: | Androsterone (3alpha-hydroxy-5alpha-androstan-17-one), 5alpha-androstane-3alpha, 17beta-diol and 5alpha-androstane-3beta, 17beta-diol were conjugated at C-16 through sulfur to bovine and human serum albumin. Rabbits injected with these conjugates produced antibodies suitable for radioimmunoassays of these hormone metabolites. Samples were purified on Sephadex LH-20 columns. Levels of these steroids were measured in a rat blood serum pool and in ovarian tissue extract pools. |
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| Keywords: | Send reprint requests to: Dr John G Wagner Upjohn Center for Clinical Pharmacology The University of Michigan Ann Arbor Michigan 48109 |
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