Expression of human histocompatibility antigens on the surface of murine cells transformed by cosmid clones containing HLA genes |
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Authors: | P. Le Bouteiller C. Foa M. Malissen P. Golstein J. R. Galindo Z. Mishal D. Caillol F. A. Lemonnier |
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Affiliation: | 1. Centre d''Immunologie INSERM-CNRS de Marseille-Luminy, 13288 Marseille Cédex 9, France;2. INSERM U. 119, 13009 Marseille, France |
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Abstract: | Thymidine kinase-negative C3H mouse L fibroblasts (LMTK−) transformed with cosmid clones containing both herpes virus-derived thymidine kinase (TK) and HLA class I genes were first selected in HAT (hypoxanthine, aminopterin, thymidine) medium and subsequently analyzed for the expression of human transplantation antigens. TK+-transformed cells expressing HLA class I molecules were characterized by surface radioimmunoassay, cytofluorimetric analysis and immunoperoxidase PAP technique at the light and electron microscopic levels, using a set of monoclonal antibodies. Comparisons were made with human B (Raji) and T (1 301) lymphoblastoid cell lines which respectively express high and low levels of HLA molecules on their surface. The expression of HLA class I in association with murine β2-microglobulin on the surface of transformed cells did not reduce the level of expression of H-2 molecules. |
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