Tomographic phase microscopy |
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| Authors: | Choi Wonshik Fang-Yen Christopher Badizadegan Kamran Oh Seungeun Lue Niyom Dasari Ramachandra R Feld Michael S |
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| Institution: | G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. |
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| Abstract: | We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering. |
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