Immobilization of lipase by salts and the transesterification activity in hexane |
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| Authors: | Wang Pu Yang Li-rong Wu Jian-ping |
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| Affiliation: | (1) School of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, P.R. China;(2) Institute of Biochemical Engineering, College of Material Science & Chemical Engineering, Yuquan Campus, Zhejiang University, Hangzhou, 310027, P.R. China |
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| Abstract: | Lipases from six different sources were immobilized on Celite and five types of salt. The transesterification activities in hexane for lipases immobilized on EDTA-Na2 increased by 463% for the lipase from Candida rugosa (CRL), 2700% for the lipase from Candida sp. (CSL) and 1215% for the lipase from Pseudomonas sp. (PSL), compared to the salt-free enzyme. With 0.5% sucrose for CRL or 1% sorbitol for PSL as the lyoprotectant during lyophilization process, transesterification activity increased by 100% and 13%, respectively, compared to the immobilized enzyme on EDTA-Na2 without lyoprotectant. |
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| Keywords: | immobilization lipase lyoprotectant salts transesterification |
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