Toxicity of argemone oil: Effect on hsp70expression and tissue damage in transgenic Drosophila melanogaster(hsp70-lacZ) Bg
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Authors: | I Mukhopadhyay A Nazir K Mahmood DK Saxena M Das SK Khanna DK Chowdhuri |
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Institution: | (1) Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, India;(2) Food Toxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India |
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Abstract: | The effect of argemone oil on hsp70expression and tissue damage was investigated by studying β-galactosidase activity, Western blotting and hybridization, and
trypan blue staining in the larval tissues of transgenic Drosophila melanogaster(hsp70-lacZ)Bg
9. Different concentrations of argemone oil were mixed with food and third-instar larvae were allowed to feed on them for different
time intervals (2, 4, 24, and 48 h). Argemone oil was found to induce hsp70even in the lowest concentration of the adulterant while maximum tissue damage was observed in the higher two treatment groups.
Malpighian tubules and midgut tissue reflected maximum damage as evidenced by both high β-galactosidase activity and trypan
blue staining in these tissues. A prior temperature shock treatment to the larvae was enough to protect the larvae from argemone
oil-induced tissue damage as evidenced by little or no trypan blue staining. The present study suggests the cytotoxic potential
of argemone oil and further strengthens the evidence for the use of hsp70as a biomarker in risk assessment.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | argemone oil cytotoxicity Drosophila melanogaster hsp70 HSP70 tissue damage Western blotting and hybridization |
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