Cloning and expression of a human muscle phosphofructokinase cDNA |
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| Authors: | P M Sharma G R Reddy S Vora B M Babior A McLachlan |
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| Institution: | Department of Molecular and Experimental Medicine, Scripps Clinic and Research Foundation, La Jolla, CA 92037. |
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| Abstract: | The nucleotide sequence of a 2.86-kb cDNA clone containing the complete human muscle phosphofructokinase (PFK) protein-coding region was determined. It comprises 76 bp of 5'-untranslated sequence, 2340 bp encoding human muscle PFK polypeptide, and 399 bp of 3'-untranslated sequence plus a poly(A) tract. A retroviral vector was utilized to express the product of this coding sequence in mouse fibroblasts. The PFK-coding cDNA was shown to code for an enzymatically active polypeptide by immunoprecipitation analysis and DEAE-Sephadex A-25 chromatography. |
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