| 油樟悬浮培养及次生代谢产物的诱导 |
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| 引用本文： | 魏琴,王丽,傅体华,周黎军. 油樟悬浮培养及次生代谢产物的诱导[J]. 植物学报, 2008, 25(5): 591-596 |
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| 作者姓名： | 魏琴 王丽 傅体华 周黎军 |
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| 作者单位： | 1宜宾学院西南特色经济植物保护与利用重点实验室, 宜宾 644000; 2四川农业大学, 雅安 625014 |
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| 摘 要： | 以油樟(Cinnamomum longepaniculatum)叶片为外植体在附加6-BA和NAA不同激素浓度组合的MS培养基上筛选质地疏松、生长旺盛的愈伤组织, 分别接种在MS、B5、WPM三种液体培养基中进行细胞悬浮培养, 并检测诱导产生的次生代谢产物。结果表明: 2 mg.L-1 6-BA+ 0.5 mg.L-1 NAA能诱导质地疏松、生长旺盛的愈伤组织, B5基本培养基中细胞长势最好; 愈伤组织在B5+2 mg.L-1 6-BA+ 0.5 mg.L-1 NAA中悬浮培养, 继代2次后形成均一的单细胞; 从油樟悬浮培养物中检测出50%以上的成分是苯甲醇。
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| 关 键 词： | 愈伤组织 油樟 次生代谢 悬浮培养 |
| 收稿时间： | 2007-12-12 |
| 修稿时间： | 2008-01-20 |
Studies of Cell Suspension Culture and Induction of Secondary Metabolism Products in Cinnamomum longepaniculatum |
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| Qin Wei,Li Wang,Tihua Fu,Lijun Zhou. Studies of Cell Suspension Culture and Induction of Secondary Metabolism Products in Cinnamomum longepaniculatum[J]. Bulletin of Botany, 2008, 25(5): 591-596 |
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| Authors: | Qin Wei Li Wang Tihua Fu Lijun Zhou |
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| Affiliation: | 1The Key Laboratory of Economic Plant Protection and Utilization in Southwest, Yibin College, Yibin 644000, China; 2 Sichuan Agricultural University, Ya'an 625014, China |
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| Abstract: | Well-growing and friable calli from leaves of Cinnamomum longepaniculatum were screened on Murashige and Skoog (MS) medium with different plant growth regulators including 6-benzyladenine (6-BA) and 1-naphthaleneacetic acid (NAA). Calli were cultured on MS medium, Gamborg medium (B5) and woody plant medium (WPM) through suspension culture, and secondary metabolism products were detected. Well-growing and friable-structured calli were induced on 2 mg.L-1 6-BA + 0.5 mg.L-1 NAA, and growth of calli was best on B5 medium in three liquid media. Single cells were formed on B5 medium with 0.2 mg.L-1 6-BA + 0.5 mg.L-1 NAA after two periods of subcultivation. More than 50% benzenemethanol was detected in the secondary metabolism products. |
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| Keywords: | ; |
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