Autoradiographic localization and characterization of angiotensin II binding sites in the spleen of rats and mice |
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| Authors: | E Castrén M Kurihara J M Saavedra |
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| Affiliation: | 3. Departments of Biological Sciences, Bridge Institute, University of Southern California, Los Angeles, California 90089;4. Departments of Chemistry, Bridge Institute, University of Southern California, Los Angeles, California 90089;5. Department of Molecular Cardiology, Lerner Research Institute of Cleveland Clinic, Cleveland, Ohio 44195;1. Howard Hughes Medical Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA;2. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA;3. Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA;1. Department of Biological Sciences, Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA;2. Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA;3. Center for Free Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany;4. Department of Chemistry, Bridge Institute, University of Southern California, Los Angeles, CA 90089 USA;5. Department of Chemistry and Biochemistry, Center for Applied Structural Discovery at the Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA;6. Department of Physics, Arizona State University, Tempe, AZ 85287, USA;7. Department of Chemistry and Biochemistry, the UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA;8. Joint Center for Structural Genomics, Stanford Synchrotron Radiation Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA;9. BioXFEL Science and Technology Center, Buffalo, NY 14203, USA;10. Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA;11. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA;12. iHuman Institute, ShanghaiTech University, Shanghai, 201210 China |
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| Abstract: | Specific binding sites for angiotensin II (Ang II) were localized in the red pulp of the spleen of rats and mice by quantitative autoradiography using 125I-Sar1-Ang II as a ligand. In the rat, the binding was saturable and specific, and the rank order for Ang II derivatives as competitors of 125I-Sar1-Ang II binding correlates well with their affinity for Ang II receptors in other tissues. Kinetic analysis in the rat spleen revealed a single class of binding sites with a KD of 1.11 nM and a Bmax value of 81.6 fmol/mg protein. Ang II binding sites were also localized on isolated rat spleen cells with similar affinity but with much lower Bmax, 9.75 fmol/mg protein. Ang II receptors were not detected in thymus sections from rats or mice, or on isolated rat thymocytes. The binding sites described here might represent a functional Ang II receptor with a role in the regulation of splenic volume and blood flow and in the modulation of the lymphocyte function. |
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