| 匙羹藤组织培养条件优化研究 |
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| 引用本文： | 苏 钛,黄宁珍,付传明. 匙羹藤组织培养条件优化研究[J]. 广西植物, 2009, 29(1): 87-91 |
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| 作者姓名： | 苏 钛 黄宁珍 付传明 |
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| 作者单位： | 广西壮族自治区中国科学院,广西植物研究所,广西,桂林,541006;广西师范大学,生命科学学院,广西,桂林,541004;广西壮族自治区中国科学院,广西植物研究所,广西,桂林,541006 |
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| 摘 要： | 以匙羹藤无菌种子苗为外植体,通过预备实验确定基本培养基后,采用完全实验和正交设计相结合的方法,研究植物生长调节剂两因素配合和多因素组合(NAA、TDZ、2-ip、IAA、6-BA和KT)对匙羹藤芽体诱导及继代培养的影响。结果显示,匙羹藤组织培养最适的基本培养基为N68;N68+NAA0.05mg/L+2-ip0.4mg/L+TDZ0.001mg/L利于芽体增殖,可用于继代培养;N68+NAA0.05mg/L+TDZ0.005mg/L促进植株长根,可用于生根培养。
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| 关 键 词： | 匙羹藤 组织培养 完全实验 正交设计 |
Optimization in vitro culture conditions for Gymnema sylvestre |
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| SU Tai,HUANG Ning-Zhen,FU Chuan-Ming. Optimization in vitro culture conditions for Gymnema sylvestre[J]. Guihaia, 2009, 29(1): 87-91 |
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| Authors: | SU Tai HUANG Ning-Zhen FU Chuan-Ming |
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| Affiliation: | 1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China; 2.College of Life Sciences, Guangxi Normal University, Guilin 541004, China |
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| Abstract: | The optimum medium for tissue culture of Gymnema sylvestre were studied by using germfree tub-seedling as explants.The effects of plant growth regulator(NAA,TDZ,2-ip,IAA,6-BA and KT) on the seedlings proliferation were studied by complete experiment and orthogonal design.The results showed that the optimum basic medium for G.sylvestre was N68;N68+NAA 0.05 mg/L+2-ip 0.4 mg/L+TDZ 0.001 mg/L was the best for bud proliferation,and can be used in subculture;N68+NAA 0.05 mg/L+TDZ 0.005 mg/L promoted rooting,can be used as rooting medium. |
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| Keywords: | Gymnema sylvestre tissue culture complete experiment orthogonal design |
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