| 催产素对大鼠背根神经节分离细胞GABA激活电流的调制作用 |
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| 作者姓名： | Tan ZJ Wei JB Li ZW Shao M Hu QS Peng BW |
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| 作者单位： | 1. 湖北医科大学生理教研室,武汉,430071
2. 同济医科大学实验医学研究中心,武汉,430030 |
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| 摘 要： | 在急性分离的大鼠背根神经节（ｄｏｒｓａｌ ｒｏｏｔ ｇａｎｇｌｉｏｎ,ＤＲＧ）细胞上,应用全细胞膜片箝技术观察了预知催产素（ｏｘｙｔｏｃｉｎ,ＯＴ）对ＧＡＢＡ激活电流的调制作用。结果如下：（１）大多数细胞（４８／５２,９０．５％）对ＧＡＢＡ敏感。（２）ＯＴ可引起５１．３％（２０／３９）的受检细胞出现外向膜电流;４３．６％（１７／３９）无明显膜反应;５．１％（２／３９）出现内向膜电流。（３）预加ＯＴ
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| 关 键 词： | 催产素 背根神经节 GABAA受体 调制 感觉信息 |
Modulation of GABA-activated currents by oxytocin in rat dorsal root ganglion neurons |
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| Tan ZJ,Wei JB,Li ZW,Shao M,Hu QS,Peng BW.Modulation of GABA-activated currents by oxytocin in rat dorsal root ganglion neurons[J].Acta Physiologica Sinica,2000,52(5):381-384. |
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| Authors: | Tan Z J Wei J B Li Z W Shao M Hu Q S Peng B W |
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| Institution: | Department of Physiology, Hubei Medical University, Wuhan 430071, China. |
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| Abstract: | Experiments were performed on freshly isolated dorsal root ganglion (DRG) neurons of rat. GABA(A)-activated currents were recorded using the whole-cell patch clamp technique. The majority of the neurons (48/52, 90.5%) were sensitive to GABA (10( 6)~10( 3) mol/L). Application of oxytocin (OT) induced outward membrane responses in 51.3% (20/39) of the neurons, no apparent responses in 43.6% (17/39) and inward responses in 5.1% (2/39). 10( 12), 10( 11), 10( 10) and 10( 9) mol/L OT increased 10( 4) mol/L GABA-activated currents to 24.1+/-7.6% (n=6), 33.4+/-6.9% (n=9), 40.2+/-6.5% (n=13) and 67.2+/-14.8% (n=5), respectively. After preapplication of OT, the Kd value for GABA(A)-activated currents decreased, while the response obtained at the maximum concentration increased. The results suggest that the enhancement of GABA-activated currents by OT may suppress primary sensory transmission by potentiating pre-synaptic inhibition of GABA. |
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| Keywords: | oxytocin dorsal root ganglion GABA A receptor whole cell patch clamp modulatio |
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