Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor |
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Authors: | L Sajc G Vunjak-Novakovic D Grubisic N Kova?evi? D Vukovi? B Bugarski |
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Institution: | (1) Department of Chemical Engineering, Faculty of Technology, Belgrade University, Karnegijeva 4, Belgrade, Yugoslavia. Fax: 381 11 322 0847, YU;(2) Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA, US;(3) Department of Plant Physiology, Faculty of Biology, Belgrade University, 29. novembra 142, Belgrade, Yugoslavia, YU;(4) Faculty of Pharmacy, Belgrade University, Vojvode Stepe 450, Belgrade, Yugoslavia, YU |
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Abstract: | The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process.
The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized
cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift
bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was
regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige
and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells
in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and
the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time
course of a batch plant cell culture. The model showed satisfactory agreement with four sets of shake flask experiments and
three bioreactor production cycles.
Received: 18 March 1994/Received revision: 20 September 1994/Accepted: 28 September 1994 |
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