|
|||||
|
|
Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells |
|
| Authors: | P V Avdonin A A Tsitrina G Y Mironova P P Avdonin I L Zharkikh A D Nadeev N V Goncharov |
| Institution: | 1.Koltzov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia;2.Institute of General Pathology and Pathophysiology Russian Academy of Medical Sciences,Moscow,Russia;3.Institute of Cell Biophysics,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia;4.Sechenov Institute of Evolutionary Physiology and Biochemistry,Russian Academy of Sciences,St. Petersburg,Russia |
| Abstract: | The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H2O2 at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of vWF on cell surface by 89 ± 5% (p < 0.01). Analysis of immunofluorescent images of HUVEC with CellProfiler program revealed that the average number of antigen positive structures on the single cell surface increases from 11.4 ± 0.16 in control up to 17.5 ± 0.21 after incubation with H2O2 (p < 0.01). vWF is exposed on the cell surface as dots with the average sizes around 1–3 μm. H2O2 causes an increase in the number of these dots and the appearence of the strings of vWF which are absent in control HUVEC. It is suggested that H2O2 may serve as a messenger which stimulates vWF exocytosis. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |