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Aroma compounds produced by Kluyveromyces marxianus in solid state fermentation on a packed bed column bioreactor
Authors:Medeiros  Adriane B.P.  Pandey  Ashok  Christen  Pierre  Fontoura  Paulo S.G.  de Freitas  Renato J.S.  Soccol  Carlos R.
Affiliation:(1) Laboratório de Processos Biotecnológicos, Departamento de Engenharia Química, Universidade Federal do Paraná (UFPR), CEP 81531-970 Curitiba-PR, Brazil;(2) Biotechnology Division, Regional Research Laboratory, Council of Scientific and Industrial Research, Trivandrum, 695-019, India;(3) Laboratoire de Microbiologie, Institut de Recherche pour le Développement, Université de Provence, Case 925, 13288 Marseille, France;(4) Laboratório de Química Analítica Aplicada, Departamento de Engenharia Química, Universidade Federal do Paraná (UFPR), CEP 81531-970 Curitiba-PR, Brazil
Abstract:Studies were carried out for the production of aroma compounds by Kluyveromyces marxianus grown on cassava bagasse in solid state fermentation using packed bed reactors, testing two different aeration rates. Respirometric analysis was used to follow the growth of the culture. Headspace analysis of the culture by gas chromatography showed the production of 11 compounds, out of which nine were identified. Ethyl acetate, ethanol and acetaldehyde were the major compounds produced. Lower aeration rate (0.06l h–1 g–1 of initial dry matter) increased total volatile (TV) production and the rate of production was also increased at this aeration rate. Using an aeration rate of 0.06l h–1 g–1 maximum TV concentrations were reached at 24 h and at 40 h with 0.12l h–1 g–1.
Keywords:Aroma compounds  cassava bagasse  Kluyveromyces marxianus  packed bed column bioreactors  solid state fermentation
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