Mapping the binding site of full length HIV-1 Nef on human Lck SH3 by NMR spectroscopy |
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Authors: | Lars Briese Andrea Preusser Dieter Willbold |
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Affiliation: | (1) Institut für Molekulare Biotechnologie, 07745 Jena, Germany;(2) IBI-2, Forschungszentrum Jülich, 52425 Jülich, Germany;(3) Institut für Physikalische Biologie, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany;(4) Present address: GE Healthcare, 79111 Freiburg, Germany;(5) Present address: Georg-August-Universität Göttingen, Abteilung Biochemie II, 37073 Göttingen, Germany |
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Abstract: | Summary The Nef protein of human immunodeficiency virus type 1 (HIV-1) is known to directly bind to the SH3 domain of human lymphocyte specific kinase (Lck) via a proline-rich region located in the amino terminal part of Nef. To address the question whether Nef binding to Lck SH3 involves residues outside the typical poly-proline peptide binding site and whether the Lck unique domain is involved in Nef–Lck interaction, we studied the direct interaction between both molecules using recombinant full-length HIV-1 Nef protein on one side and recombinantly expressed and uniformly 15N-isotope labeled Lck protein comprising unique and SH3 domains on the other side. Applying nuclear magnetic resonance spectroscopy we could show that only residues of Lck SH3, that are typically involved in binding poly-proline peptides, are affected by Nef binding. Further, for the first time we could rule out that residues of Lck unique domain are involved in binding to full length Nef protein. Thus, interactions of Lck unique domain to cellular partners e.g. CD4 or CD8, are not necessarily competitive with Lck binding to HIV-1 Nef. |
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Keywords: | chemical shift mapping human immunodeficiency virus Lck ligand binding Nef nuclear magnetic resonance spectroscopy protein– protein interaction protein structure |
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