Enhancement of analyte ionization in desoprtion/ionization on porous silicon (DIOS)-mass spectrometry (MS) |
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Authors: | Chang-Soo Lee Eun-Mi Kim Sang-Ho Lee Min-Soo Kim Yong-Kweon Kim Byug-Gee Kim |
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Institution: | (1) Department of Chemical Engineering, Chungnam National University, 305-764 Daejeon, Korea;(2) School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University, 151-744 Seoul, Korea;(3) School of Electrical Engineering and Computer Science, Seoul National University, 151-744 Seoul, Korea |
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Abstract: | Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a relatively new laser desorption/ionization technique for
mass spectrometry without employing an organic matrix. This present study was carried to survey the experimental factors to
improve the efficiency of DIOS-MS through electrochemical etching condition in structure and morphological properties of the
porous silicon. The porous structure of silicon structure and its properties are crucial for the better performance of DIOS-MS
and they can be controlled by the suitable selection of electrochemical conditions. The fabrication of porous silicon and
ion signals on DIOS-MS were examined as a function of silicon orientation, etching time, etchant, current flux, irradiation,
pore size, and pore depth. We have also examined the effect of pre- and post-etching conditions for their effect on DIOS-MS.
Finally, we could optimize the electrochemical conditions for the efficient performance of DIOS-MS in the analysis of small
molecule such as amino acid, drug and peptides without any unknown noise or fragmentation. |
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Keywords: | porous silicon desorption/ionization on silicon (DIOS) matrix assisted laser desorption/ionization (MALDI) small molecule analysis |
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