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A two-dimensional polyacrylamide gel electrophoresis (PAGE) system using sodium dodecyl sulfate-PAGE in the first dimension. |
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| Authors: | G P Tuszynski C A Buck L Warren |
| Institution: | The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania 19104, USA |
| Abstract: | A two-dimensional gel electrophoretic system for the separation of cellular proteins is described. The system utilizes sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in the first dimension and polyacrylamide gel isoelectric focusing in the second dimension. The system offers a good starting point for many difficult protein separations requiring SDS. |
| Keywords: | SDS sodium dodecyl sulfate PAGE polyacrylamide gel electrophoresis PAGIF polyacrylamide gel isoelectric focusing PMSF phenylmethylsulfonyl fluoride NP-40 Nonidet P40 BIS TEMED baby hamster kidney fibroblasts clone 13 PBS phosphate buffered saline BSA bovine serum albumin |
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