茶多酚干预乳腺癌组织及重要器官血管生成相关因子表达研究

目的:观察茶多酚对移植性小鼠乳腺癌(EMT_6)组织与重要脏器(心、脑、肾)组织血管生成相关因子表达影响。方法:应用小鼠可移植性乳腺癌EMT_6细胞株,经培养传代后,以纯系BALB/c小鼠为荷瘤动物进行移植,并采用茶多酚灌胃及局部注射两种干预措施,以免疫组化方法检测小鼠乳腺癌组织VEGF、bFGF、TIMP-2表达,并测定心、脑、肾组织中VEGF及TIMP-2表达。结果:与模型对照组比较,茶多酚两种给药途径的肿瘤组织VEGF、bFGF阳性表达明显降低(P<0.05);TIMP-2阳性表达明显增高(P<0.05);而心、脑、肾组织VEGF、TMP-2阳性表达无明显差异(P>0.05)。结论:茶多酚可明显抑制新生血管生成相关因子表达,并特异性的作用于肿瘤靶点部位,预示在肿瘤治疗领域具有广泛的应用前景。

The Effect of Tea Polyphenols on Expression of Tumor Blood Vessels Related Factors in Tumor Tissues and Vital Organs of Implanted Breast Cancer

Objective:To observe the effect of tea polyphenols on expression of tumor blood vessels related factors in tumor tissue and vital organs(such as heart,brain and kidney)on implanted mouse breast cancer(EMT_6).Methods:Mammary adenocarcinoma EMT_6 was cultured and propagated,then implanted on female BALB/c mice.Tea polyphenols was peroral administrated and local injected,then VEGF,bFGF,TIMP-2 in tumor tissue and VEGF,TIMP-2 in vital organs(heart,brain and kidney)was detected by immunohistochemical method.Results:Oral administration and local injection of tea polyphenols on implanted breast cancer mice could markedly reduce expression of VEGF,bFGF and increase expression of TIMP-2 in tumor tissue(P<0.05),while have no significance effect on vital organs(P>0.05).Conclusions:Tea polyphenols can inhibit the expression of vessels related factors,selectively aim at tumor tissue and have no significant effect on vital organs such as heart,brain and kidney.This result offer a broad prospect in clinical application in oncotherapy field.