A rapid assay for 2',3'-cyclic nucleotide 3'-phosphohydrolase.

Abstract— Separation of 2′-adenosine monophosphate from 2′,3′-cyclic adenosine monophosphate by coprecipitation with a number of salt mixtures was examined and found to be most successful with Na₂CO₃/CdCl₂ and Na₂CO₃/ZnSO₄. A simple and rapid assay for 2′,3′-cyclic nucleotide 3′-phosphohydrolase using coprecipitation with Na₂CO₃/CdCl₂ is described.