Presence and cellular distribution of soybean agglutinin-binding epitopes in two strains of<Emphasis Type="Italic">Bacillus subtilis</Emphasis> |
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Authors: | S R Stoitsova |
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Institution: | (1) Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sojia, Bulgaria |
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Abstract: | The cellular location ofN-acetylgalactosamine inBacillus subtilis strains 168 and 170 was examined by electron microscopy using gold-conjugated soybean agglutinin (SBA) as a marker. Post-embedding
labeling of sectioned material showed SBA-reactive, galactosamine-containing polymers associated with the cell membrane or
the cytoplasm of the two strains. This intracellular location was distinct from the concanavalin A-binding epitopes that were
located over the cell wall. Labeling of whole cells (native, fixed in glutaraldehyde, or treated with proteinase K, or Tween
20) before negative staining revealed no galactosamine exposed on the surface of strain 168. On the surface of strain 168
some exposed galactosamine terminal residues were detected; their accessibility to SBA increased when Tween 20 or proteinase
K was applied. |
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Keywords: | |
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