Plasmid vectors for protein production, gene expression and molecular manipulations in Aspergillus niger |
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Authors: | Storms Reginald Zheng Yun Li Hongshan Sillaots Susan Martinez-Perez Amalia Tsang Adrian |
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Institution: | Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Que., Canada H4B 1R6. storms@vax2.concordia.ca |
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Abstract: | We constructed three sets of plasmids for use in Aspergillus niger. These plasmids were assembled using various combinations of a series of modular DNA cassettes that included a selectable marker, pyrG, derived from Aspergillus nidulans; two promoter regions for directing protein expression; a cassette derived from the AMA1 replicator sequence to support autonomous replication; and a reporter gene based on the A. niger lacA gene. One set included integrating and autonomously replicating plasmids for the expression of homologous and heterologous proteins. The second was a set of autonomously replicating plasmids, with a secreted beta-galactosidase encoding reporter gene, for studying gene regulation events. The third set included pyrG-derived gene-blaster cassettes suitable for genome manipulation by targeted gene replacement. |
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